COMPARATIVE ACTIVITY OF ANTIBIOTICS AND BACTERIOPHAGES AGAINST COMMUNITY-MADE STRAINS OF PSEUDOMONAS AERUGINOSA

The article is sanctified to the study of activity of antibiotics and bacteriophages in relation to community-made strains of Pseudomonas aeruginosa. Pseudomonas aeruginosa, as a typical species of the genus Pseudomonas, is known in the territories of all continents, since, on the one hand, this stick is an ordinary inhabitant of reservoirs, various plant substrates, and on the other hand, it exhibits pathogenic properties in relation to various objects of nature, people, warm blooded animals, arthropods, and the like. Due to the peculiarities of its ecology, the widespread use of the Pseudomonas spider was due to the lack of requirements for organic matter for life, the ability to persistence in a non-culturally active state and in the composition of bacterial biofilms, a wide range of resistance to various environmental factors: antibiotics, bacteriophages, disinfectants, and antiseptics. In spite of the achievement of microbiology and practical medicine, the problems of Pseudomonas aerobic infection, both in clinical practice and in veterinary medicine, remain relevant and diverse, include issues of diagnosis, determination of the pathogenic potential of the pathogen, choice of effective treatment tactics, advanced preventive measures, etc. In modern conditions, when the monopoly position of antibiotics in the complex of antimicrobials is called into question, scientific researches aimed at increasing the efficiency of bacteriophagotherapy become of increasing importance. For experimental studies, we used commercial bacteriophages: Pseudomonas bacteriophage (FGUP NPO Microgen, Perm.) and Piobacteriophage (FSUE NPO Microgen MH RF, Nizhny Novgorod). The sensitivity to specific bacteriophages was determined by the drop method. For the experiment, a suspension of daily agar culture was prepared in an isotonic sodium chloride solution, which was applied to the nutrient agar according rule, after the phage addition. Most strains of P. aeruginosa, which were taken at a concentration of 109 to 107 CFU / ml for the determination of their sensitivity to the bacteriophages, were assigned to a persistent or weakly sensitive ( - or + , according to the generally accepted evaluation scheme) up to 106 - 104 CFU / ml - became medium-sensitive (on ++ - +++ ). Particular importance for adsorption of phage to a bacterial cell (the initial and most important stage of interaction), has the pH value of the medium. It should be in parameters 7-10. Adsorption occurs only on a