Ekstrak Air Daun Padi Memperbaiki Hematopoesis pada Tikus yang Terpajan Plumbum

Plumbum ( Pb ) acetate leads to hematopoiesis disruption by inhibiting heme biosynthesis and creating G-6PD enzyme deficiency, leading to decreased hemoglobin concentration and red cell count as well as increased reticulocyte cells. Rice leaf extract contains metallothionein proteins that bind Pb. This study aimed to prove the ability of rice leaf extract wa to improve hematopoiesis disorder conditions in rats exposed to Pb. The method used was the randomized post-test only control group design. Twenty eight rats were divided into 4 groups: 1 control group and 3 treatment groups . In the control group and the treatments groups, the experimental rats were treated with 0.5 g/kg/day Pb. The treatment groups were treated by 0.2, 0.4, and 0.8 rice leaf extract, respectively, every day for 8 weeks. On the last day of the 8th week, blood samples were collected for Hb concentration measurements using cyanmethemoglobin and the erythrocytes and reticulocytes number were counted using a hematology analyzer and BCB, respectively. This study was conducted in LPPT UGM and Biomedicine UNIMUS in March–May 2014. The differences between groups in hemoglobin and erythrocyte parameters were tasted using one way ANOVA while Kruskal-Wallis test was used for the reticulocyte count. The results show that rice leaf extract treatment to rats exposed to Pb did not affect Hb (p=0.81) and erythrocytes count (p=0.83), However, the mean reticulocyte count decreased significantly in Pb-exposed rats treated with rice leaf extract (p=0.00). In conclusion, rice leaf extract is able to improve hematopoiesis in rats exposed to Pb.