FREQUENCY OF PROTHROMBIN GENE MUTATION IN VENOUS THROMBOEMBOLISM IN NORTHERN PAKISTAN

According to western published data, annual incidence of first episode of venous thrombosis is 1.5 per 1000 person/year with a per person life time incidence of 5%3. Patients with 20210G>A mutation frequently show plasma prothrombin levels three times higher than normal by increasing mRNA and protein expression for prothrombin. [...]increased prothrombin levels leads to an increase in a protein called thrombin-activatable fibrinolysis inhibitor (TAFI), which is an inhibitor of the fibrinolysis. [...]an increase in TAFI may disturb process of fibrinolysis allowing accumulation of clots leading to VTE10. The DNA was amplified in a 20pl reaction mixture in an ependorff tube containing 20 pmol each of forward and reverse primers, 0.5 units of Taq polymerase (Fermentas life sciences, Lithuania), PCR master mix containing (30 mmol of each dNTP, 10 m mol tris HCl (pH 8.3), 50 mmol KCL, 2.0 mmol MgCl2, 10 mg/ml gelatin and 0.1-0.3 mg of genomic DNA.