Root-hypoxia tolerance in soybean sister-lines plants indicates a better balance in energy use/dissipation and oxidative stress control

•Results obtained by cultivation simulated at field conditions.•Long exposure flooding did not negatively affect the tolerant sister line.•Tolerant sister line presents a rapid metabolic change to cope hypoxia effect.•Both sister lines coped with oxidative stress, differently. Climatic changes are leading to an increased number of flooding year by year which impacts negatively the metabolism of roots and influences the shoot metabolism which leads to a decline in soybean productivity. This can be more drastic when it is cultivated in lowland soils. Our group hypothesized that tolerant sister-line plants have more capacity to cope with oxidative stress due to better management of energy use and dissipation on photosynthesis machinery during root-flooding and recovery conditions, while in sensitive soybean sister-line the impairment of the photosynthetic use and dissipation of energy lead to an unbalanced redox state response. For that, soybean seeds from the same parents, PELBR15- 7015C (flooding tolerant) and PELBR15-7060 (flooding sensitive) were sowed and cultivated in 500 L capacity plastic containers with lowland soil simulating the field management process. At the reproductive stage, the roots were subjected to flooding for 11 days, then drained to analyze reoxygenation (3 d) and recovery (10 d). Tolerant plants have a greater ability to manage root flooding conditions compared to sensitive ones. The ability includes efficient energy use and dissipation, which minimizes the production of reactive oxygen species (ROS) and subsequent cell damage. Conversely, in sensitive sister lines, impaired electron transport chains lead to increased ROS production and damage. Upon reoxygenation and recovery, the tolerant sister lines exhibit a faster metabolism turnover, allowing them to cope more effectively with the stress on leaves and roots. While both sister lines are capable of detoxifying ROS, the tolerant sister line's superior ability to manage energy use and dissipation allows for more stable and rapid control of oxidative stress.