Behaviour of the Sydney funnel-web spider Atrax robustus over different contexts, time, and stimuli

Atrax robustus is an iconic Australian spider because the venom can be lethal to humans. Moreover, some of the venom biomolecules have promise as therapeutic and bioinsecticidal leads. Nonetheless, aspects related to the life history and behaviour of this species, which might influence changes in venom components, have been overlooked. We assessed different behavioural traits (antipredator behaviour, defensiveness and activity) of juveniles and adult females across different contexts (predation, conspecific tolerance and exploration of a new territory) and stimuli (puff of air versus prod) over time. Adults responded to a puff of air faster than juveniles, but in response to a prod, both juveniles and adults become more defensive over time. No differences were observed between adults and juveniles for conspecific tolerance and exploration. Understanding behaviour of venomous species is important because behaviours may affect physiological traits, such as venom, and the ability of spiders to adapt to different conditions. [Display omitted] •Study of Sydney funnel-web spiders behaviour in response to different stimuli over time and different contexts.•Adults and juveniles show different behavioural responses to an aversive stimulus.•Adults show flexibility of aggressive behaviour in response to a threatening stimulus.•The type of threatening stimulus affects the way spiders modulate their behaviour.