The Effect of Temperature on Carp (Cyprinus Carpio, L., 1758) Microbiota, Reared in Intensive Conditions, by Using the BioPlus® 2B Probiotic

A 60-days experimental trial was conducted to examine the influence of probiotic BioPlus® 2B, in different concentration, on the gut microbial load and the organic load of technological water used for carp growth, in low water temperature (13.5-16.8oC) conditions. Four variants were tested, as follows: V1-pellets with 30% crude protein, without probiotics; V2-pellets with 30% crude protein, with probiotics of 2.24×109 CFU/kg food; V3-pellets with 30% crude protein, with probiotics of 3.84×109 CFU/kg food; and V4-pellets with 30% crude protein, with probiotics of 7.04×109 CFU/kg food. BioPlus® 2B probiotics (a mixture of Bacillus licheniformis (DSM 5749) and Bacillus subtilis (DSM 5750) were used. In order to determine the total number of germs (NTG), microbiological analyses were made. As a result, it can be stated that during the first experimental stage, gut microbial load had registered a significant increase (p