Comparative genomics of multidrug-resistant Enterococcus spp. isolated from wastewater treatment plants

Wastewater treatment plants (WWTPs) are considered hotspots for the environmental dissemination of antimicrobial resistance (AMR) determinants. Vancomycin-Resistant Enterococcus (VRE) are candidates for gauging the degree of AMR bacteria in wastewater. Enterococcus faecalis and Enterococcus faecium...

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Veröffentlicht in:	BMC microbiology 2020-01, Vol.20 (1), p.20-17, Article 20
Hauptverfasser:	Sanderson, Haley, Ortega-Polo, Rodrigo, Zaheer, Rahat, Goji, Noriko, Amoako, Kingsley K, Brown, R Stephen, Majury, Anna, Liss, Steven N, McAllister, Tim A
Format:	Artikel
Sprache:	eng
Schlagworte:	Activated sludge Aeration Antibiotic resistance Antibiotics Antimicrobial agents Antimicrobial resistance Arrays Bacterial Proteins - genetics Biomarkers Biosynthesis Cell division Clustering Comparative genomic hybridization Contamination CRISPR Drug resistance Drug Resistance, Multiple, Bacterial Efflux Enterococci Enterococcus Enterococcus faecium - genetics Environmental aspects Fecal coliforms Food Gaging Genes Genetic aspects Genome Size Genomes Genomics Genomics - methods Harbors Infections Interspersed Repetitive Sequences Metabolism Metabolites Methods Microbiological research Mobilome Motility Multidrug resistance Multidrug resistant organisms Multilocus Sequence Typing Observations Phages Phenotypes Phylogeny Sewage disposal plants Signal transduction Vancomycin Vancomycin Resistance Vancomycin resistant Enterococcus (VRE) Virulence Virulence Factors - genetics Wastewater - microbiology Wastewater treatment Wastewater treatment plants Water pollution Water treatment Whole Genome Sequencing
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Zusammenfassung:	Wastewater treatment plants (WWTPs) are considered hotspots for the environmental dissemination of antimicrobial resistance (AMR) determinants. Vancomycin-Resistant Enterococcus (VRE) are candidates for gauging the degree of AMR bacteria in wastewater. Enterococcus faecalis and Enterococcus faecium are recognized indicators of fecal contamination in water. Comparative genomics of enterococci isolated from conventional activated sludge (CAS) and biological aerated filter (BAF) WWTPs was conducted. VRE isolates, including E. faecalis (n = 24), E. faecium (n = 11), E. casseliflavus (n = 2) and E. gallinarum (n = 2) were selected for sequencing based on WWTP source, species and AMR phenotype. The pangenomes of E. faecium and E. faecalis were both open. The genomic fraction related to the mobilome was positively correlated with genome size in E. faecium (p
ISSN:	1471-2180 1471-2180
DOI:	10.1186/s12866-019-1683-4