Rapid Isolation of Functional ex vivo Human Skin Tissue-Resident Memory T Lymphocytes

Studies in animal models have shown that skin tissue-resident memory T (T ) cells provide enhanced and immediate effector function at the site of infection. However, analyses of skin T cells in humans have been hindered by the lack of an optimized isolation protocol. Here, we present a combinatorial...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Frontiers in immunology 2021-03, Vol.12, p.624013-624013
Hauptverfasser: Du, Weijie, Lenz, Daniel, Köhler, Ralf, Zhang, Erping, Cendon, Carla, Li, Jinchan, Massoud, Mona, Wachtlin, Joachim, Bodo, Juliane, Hauser, Anja E, Radbruch, Andreas, Dong, Jun
Format: Artikel
Sprache:eng
Schlagworte:
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:Studies in animal models have shown that skin tissue-resident memory T (T ) cells provide enhanced and immediate effector function at the site of infection. However, analyses of skin T cells in humans have been hindered by the lack of an optimized isolation protocol. Here, we present a combinatorial strategy-the 6-h collagenase IV digestion and gentle tissue dissociation - for rapid and efficient isolation of skin T cells with skin tissue-specific immune features. In comparison with paired blood circulating memory T cells, these isolated skin T cells express typical T cell markers and display higher polyfunctional properties. Moreover, these isolated cells can also be assessed for longer periods of time in cultures. Thus, the optimized isolation protocol provides a valuable tool for further understanding of human skin T cells, especially for direct comparison with peripheral blood T cells at the same sample collection time.
ISSN:1664-3224
1664-3224
DOI:10.3389/fimmu.2021.624013