Method of Accelerated Evaluation of Antimicrobial Preparation Efficacy against Experimental Brucellosis

Objective of the study was to elaborate the method for preliminary accelerated evaluation of antimicrobial preparation efficacy in case of in vivo brucellosis. Materials and methods. Outbred BALB/c mice with the acute septic experimental brucellosis, caused by intravenous inoculation with sub-lethal doses of the agent, served as biological models. Concurrently with the challenge, animals were given antimicrobial treatment. They took preparations of different types. Further, against the background of antimicrobial therapy, changes of quantitative microbe content in the internal organs of animals were studied, applying bacteriological method. Based on the data obtained index of antimicrobial activity was calculated, according to the value of which, it is possible to rank the drugs under study. Results and discussion. Results of preparation potency estimation, received with the help of the developed method, and results obtained after complete standard regimens show high-level correlation dependence (correlation ratio being 0.73), which makes the method suitable for screening of new antimicrobials in reference to brucellosis agent.