Dual effects of zearalenone on aflatoxin B1–induced liver and mammary gland toxicity in pregnant and lactating rats

Food and feed are frequently co-contaminated with aflatoxin B1 (AFB1) and zearalenone (ZEN). This study investigated the effects of ZEN on the AFB1–induced liver and mammary gland toxicity in pregnant and lactating rats. AFB1 and ZEN co-exposure inhibited the growth of rats and caused oxidative stress and inflammatory responses in the liver and mammary gland. Compared with the AFB1-only group, damage was aggravated in the AFB1 + 10 mg/kg ZEN group, and the AFB1 + 1 mg/kg ZEN group showed a reduction in some metrics. The metabolomic results of the mammary gland showed that metabolite changes were mainly in lipid, amino acid, and glucose metabolism. Compared with the AFB1 + 0 mg/kg ZEN group, the AFB1 + 1 mg/kg ZEN group had the most metabolite changes. Moreover, AFB1 and ZEN co-exposure reduced the levels of sex hormones and RNA m6A methylation in the mammary gland. We speculate that ZEN affects the toxicity of AFB1 to the liver and mammary gland by interfering with the function of sex hormones, regulating cell proliferation and metabolic processes. [Display omitted] •Aflatoxin B1 and zearalenone impair rat growth during pregnancy and lactation.•Zearalenone enhances the toxicity of aflatoxin B1 in liver and mammary gland.•The altered metabolites are mostly lipids, amino acids, and carbohydrates.•Aflatoxin B1 and zearalenone increase the risk of mastitis and breast cancer.