Association between ambient air pollutants and birthweight of singletons following assisted reproductive technologies

To investigate the associations between exposure to ambient air pollutants and birthweight following ART treatment. Retrospective cohort study. We included 11,599 singletons derived from fresh cycles or frozen-thawed embryo transfer (FET) cycles between Jan 2013 and Dec 2019. Exposure to six air pollutants (SO2, NO2, CO, O3, PM2.5, and PM10) at patients` residences and the clinic site were estimated using the inverse distance weighting interpolation method based on data obtained from monitor sites. The daily mean levels of pollutants were estimated in potential exposure windows (the period from three months before treatment to oocyte retrieval, the period of ovarian stimulation, the period of in vitro culture, the period from embryo transfer to hCG test, the period of entire pregnancy, the 1st, 2nd, and 3rd trimester) were calculated. Generalized additive models adjusted for confounders including maternal age, BMI, and parity were used to evaluate the association between exposures and birthweight. Interaction of exposures and ART-associated factors, such as supraphysiologic estradiol and frozen-thawed, were explored in an XGboost model. Birthweight and z-score of singletons. In fresh cycles, O3 exposure during the period from three months before treatment to oocyte retrieval and SO2 exposure during in vitro culture at the ART clinic showed a linear association with birthweight (7.24, 95% CI: 1.18–13.31 g per 10 μg/m3 increase in O3; 25.92, 95% CI: 8.26–43.58 g per 10 μg/m3 increase in SO2, respectively). For patients receiving single blastocyst transfer with exposures below the China standard of 20 μg/m3, an increase of 10 μg/m3 in SO2 was associated with a 61.52 (95% CI: 1.13–121.91) g increase in birthweight. In FET cycles, no significant association was found between air pollution and birthweight. XGboost model did not reveal a strong interaction between the exposures and ART-related factors, except for the interactions between O3 exposure and BMI. However, none of the interactions reached a higher rank of importance. Air pollution exposure during ART treatment may affect the birthweight of the offspring. [Display omitted] •SO2 exposure at the IVF site may affect birth weight following ART.•O3 exposure during follicular development is positively associated with birth weight.•Air pollution affects birth weight at levels below the WHO guideline values.•Interaction between O3 exposure and BMI was revealed by an XGboost model.