1514 In vivo CAR-M: redirecting endogenous myeloid cells with mRNA for cancer immunotherapy

BackgroundMacrophages, monocytes, and dendritic cells are sentinel cells of the innate immune system that play a central role in phagocytosis, inflammation, immune cell recruitment, and antigen presentation. Genetically redirecting myeloid cells against tumor-associated antigens represents a novel strategy for cancer immunotherapy. Ex vivo chimeric antigen receptor (CAR) macrophage and monocyte cell therapies have demonstrated robust anti-tumor immunity via targeted phagocytosis, cytokine/chemokine release, activation of the tumor microenvironment (TME), T cell recruitment, and epitope spreading in pre-clinical models. Here, we describe a novel strategy to deliver modified messenger RNA (mRNA) encapsulated in lipid nanoparticles (LNPs) to generate CAR-M (macrophages and monocytes) directly in vivo.MethodsHuman and murine primary monocytes and macrophages were utilized to characterize mRNA/LNP driven CAR expression kinetics and anti-tumor functionality in vitro using flow cytometry, live cell imaging, single cell RNA sequencing (scRNAseq), and cytokine release assays. The efficacy, safety, and myeloid cell tropism of CAR-encoding mRNA/LNP were evaluated in vivo in syngeneic and humanized mouse models of solid tumors. Tissues were collected and CAR expression along with dynamic changes in immune cell frequency and phenotype were assessed using flow cytometry and scRNAseq. All animal studies were conducted in accordance with the Animal Welfare Act and Public Health Service Policy on Humane Care and Use of Laboratory Animals and approved by the Institutional Animal Care and Use Committee at the Wistar Institute.ResultsHuman macrophages and monocytes engineered with CAR-encoding mRNA/LNP in vitro demonstrated high CAR expression and viability. CAR expression conferred antigen specificity leading to target-specific proinflammatory cytokine secretion and tumor cell killing, with serial killing demonstrated upon tumor rechallenge. The myeloid tropism of the LNP was demonstrated both in vitro and in vivo, with significant CAR expression observed in macrophages, monocytes, and dendritic cells compared to immune cells of non-myeloid origin in mice. In vivo, regional and systemic administration of CAR-encoding mRNA/LNP led to significant tumor regression in subcutaneous and systemically disseminated metastatic solid tumor models, respectively. Treated animals tolerated repeat mRNA/LNP administration with no signs of toxicity.ConclusionsThese data demonstrate that CAR-