A 7-Hydroxybenzoxazinone-Containing Fluorescence Turn-On Probe for Biothiols and Its Bioimaging Applications

In this work, a novel 7-hydroxybenzoxazinone-based fluorescent probe ( ) for the selective sensing of biothiols is reported. Upon treatment with biothiols, shows a strong fluorescence enhancement (up to 70-fold) and a large Stokes shift (155 nm). Meanwhile, this probe exhibits high resistance to int...

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Veröffentlicht in:	Molecules (Basel, Switzerland) Switzerland), 2019-08, Vol.24 (17), p.3102
Hauptverfasser:	Li, Bin, Zhang, Datong, An, Ruibing, Zhu, Yaling
Format:	Artikel
Sprache:	eng
Schlagworte:	2,4-dinitrobenzenesulfonate 7-hydroxy-3-phenyl-benzoxazinone Amino acids Benzoxazines - chemistry Biological activity Biosensing Techniques - methods biothiols Chemical compounds Cysteine - analysis Dyes Fluorescence fluorescence probe Fluorescent Dyes - chemistry Fluorophores Glutathione - analysis HeLa Cells Homocysteine Homocysteine - analysis Humans Hydrogen peroxide large Stokes shift Limit of Detection Medical imaging Optical Imaging Salts Selectivity Sodium chloride Sodium dithionite Sodium sulfate Sodium sulfide Sulfur
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Beschreibung
Zusammenfassung:	In this work, a novel 7-hydroxybenzoxazinone-based fluorescent probe ( ) for the selective sensing of biothiols is reported. Upon treatment with biothiols, shows a strong fluorescence enhancement (up to 70-fold) and a large Stokes shift (155 nm). Meanwhile, this probe exhibits high resistance to interference from other amino acids and competing species. features good linearity ranges with a low detection limit of 14.5 nM for glutathione (GSH), 17.5 nM for cysteine (Cys), and 80.0 nM for homocysteine (Hcy), respectively. Finally, the potential utility of this probe for biothiol sensing in living HeLa cells is demonstrated.
ISSN:	1420-3049 1420-3049
DOI:	10.3390/molecules24173102