Kinetic determination of morin nanoamounts

kinetic-spectrophotometric method is proposed for the determination of morin. The method is based on the inhibition effect of morin on the oxidation of C6H5COON aby hydrogen peroxide in the presence of the complex Fe(II)-AA(ascorbic acid),which acts as a catalyst. The concentration range for the determination of morin is one of the lowest achieved so far (a linear calibration graph was obtained for morin from 2.255?22.55 ng cm-3). The limit of detection of the method is 0.28 ng cm-3. The relative error ranges between 1.42 to 5.10% for the given concentration interval. Kinetic equations are proposed for the investigated process. The effects of certain foreign ions upon the reaction rate were determined in order to assess the selectivity of the method. The major advantages of this kinetic-spectrophotometric assay are its sensitivity, selectivity, reproducibility, speed and simplicity. Predlozena je nova kineticko-spektrofotometrijska metoda za odredjivanje morina. Metoda se zasniva na inhibicionom efektu morina na oksidaciju natrijum benzoata vodonik- peroksidom u kiseloj sredini u prisustvu kompleksa Fe(II)-AA(askorbinska kiselina), koji ima ulogu katalizatora. Granica detekcije je 0,28 ng cm?3. Relativna greska metode se krece od 1,42?5,1%za koncentracioni interval u opsegu od 2,255?22,55 ng cm?3. Postavljene su kineticke jednacine za ispitivane procese. Radi ocene selektivnosti ispitan je uticaj veceg broja stranih jona i organskih molekula na brzinu reakcije. Reakcija je visoko selektivna prema neorganskim anjonima i katjonima. Glavne karakteristike ove metode su visoka osetljivost, selektivnost reproduktivnost, brzina izvodjenja i jednostavnost.