Intron Regions as Genetic Markers for Population Genetic Investigations of Opisthorchis viverrini sensu lato and Clonorchis sinensis

Opisthorchiasis and clonorchiasis are prevalent in Southeast and Far-East Asia, which are caused by the group 1 carcinogenic liver flukes sensu lato and infection. There have been comprehensive investigations of systematics and genetic variation of these liver flukes. Previous studies have shown that is a species complex, called " sensu lato". More comprehensive investigations of molecular systematics and population genetics of each of the species that make up the species complex are required. Thus, other polymorphic genetic markers need to be developed. Therefore, this study aimed to characterize the intron regions of taurocyamine kinase gene (TK) to examine the genetic variation and population genetics of and collected from different geographical isolates and from a range of animal hosts. We screened seven intron regions embedded in TK. Of these, we selected an intron 5 of domain 1 (TkD1Int5) region to investigate the genetic variation and population genetics of theses liver flukes. The high nucleotide and haplotype diversity of TkD1Int5 was detected in . Heterozygosity with several insertion/deletion (indel) regions were detected in TkD1Int5 of the samples, whereas only an indel nucleotide was detected in one sample. Several samples contained three different haplotypes within a particular heterozygous sample. There were no genetic differences between isolated from various animal host. Heterozygous patterns specifically detected in humans was observed in . Thus, TkD1Int5 is a high polymorphic genetic marker, which could be an alternative marker for further population genetic investigations of these carcinogenic liver flukes and other related species from a wide geographical distribution and variety of animal hosts.