Improved selection medium and technique for isolation of Azospirillum irakense

An improved selection medium and technique for Isolation of Azospirillum irakense was developed . This technique is based on the utilization of free-nitrogensemi-solid medium supplement with disaccharide, lactulose, as a sole source of carbon and energy instead of malate with a pH value of 7.4 .This selected medium was found to encourage the growth of A. irakense alone and to prevent the other species of Azospirillum.The improved medium was superior than the other available media .The technique was simply applied to detect A.irakense, showed a high increase in numbers of A.irakense isolates obtaining from the soil rhizosphere and roots, and decreased the time cost