Application of non-directional screening technology for plant-derived components in food

ObjectiveTo provide technical support for combating adulteration of plant-derived ingredients， the detection application of non-directional screening of plant-derived ingredients in food was realized.MethodsModified CTAB， SDS， and kit methods were used to extract genomic DNA from various plant-derived foods. Quality， concentration， and purity of the obtained DNA were compared. Using the RbcL gene in the chloroplast genome of plants as a barcode， 30 plant species were selected to verify the universality and specificity of this primer. Combining the second-generation high-throughput sequencing with DNA barcode technology， species identification of four mixed plant samples sold in Shandong Province was performed.ResultsDNA extraction using the improved CTAB and kit methods removed the interference of polysaccharides and polyphenols， which was an ideal effect， whereas that of the SDS method was poor. The DNA concentration obtained by CTAB method was higher than that obtained using the kit method. This method had