Changes in behavioral priority influence the accessibility of working memory content

•We decoded high- and low-priority working memory representations from EEG recordings obtained while human volunteers performed a retrospectively cued WM task.•Probabilistic changes in the behavioral relevance of a remembered item had no effect on our ability to decode it from EEG signals; instead, these changes influenced the latency at which above-chance decoding performance was reached.•The results indicate that probabilistic changes in the behavioral relevance of WM content influence the ease with which memories can be accessed and independently of memory strength. Evolving behavioral goals require the existence of selection mechanisms that prioritize task-relevant working memory (WM) content for action. Selecting an item stored in WM is known to blunt and/or reverse information loss in stimulus-specific representations of that item reconstructed from human brain activity, but extant studies have focused on all-or-none circumstances that allow or disallow an agent to select one of several items stored in WM. Conversely, behavioral studies suggest that humans can flexibly assign different levels of priority to different items stored in WM, but how doing so influences neural representations of WM content is unclear. One possibility is that assigning different levels of priority to items in WM influences the quality of those representations, resulting in more robust neural representations of high- vs. low-priority WM content. A second – and non-exclusive – possibility is that asymmetries in behavioral priority influence how rapidly neural representations of high- vs. low-priority WM content can be selected and reported. We tested these possibilities in two experiments by decoding high- and low-priority WM content from EEG recordings obtained while human volunteers performed a retrospectively cued WM task. Probabilistic changes in the behavioral relevance of a remembered item had no effect on our ability to decode it from EEG signals; instead, these changes influenced the latency at which above-chance decoding performance was reached. Thus, our results indicate that probabilistic changes in the behavioral relevance of WM content influence the ease with which memories can be selected independently of their strength.