Effect of chicken egg shell powder solution on early enamel carious lesions: an invitro preliminary study

To evaluate the remineralization potential of enamel surface lesion using chicken eggshell powder (CESP) solution. Ten freshly extracted un-erupted third molars decoronated at cement-enamel junction (CEJ) used in this study. Each decoronated tooth was sectioned to get 4 samples of enamel blocks such that 40 blocks were obtained which were then subjected to demineralization protocol and grouped as: Group 1-untreated group, Group 2-subsurface demineralization, Group 3-subsurface demineralization + 7 days CESP immersion, Group 4- subsurface demineralization + clinpro application. The samples were evaluated for X-ray fluorescence spectroscopy analysis, microhardness testing and atomic analyses using energy dispersive X-ray spectroscopy. The data were statistically analysed by using one-way ANOVA and Tukey - Kramer multiple comparison test. X-ray Fluorescence spectroscopy shows Calcium concentration of 98% and 0.46% of phosphate. Group 4 (Clinpro) shows the highest efficacy in enhancing the remineralization followed by Group 3 (CESP). The results of atomic analyses showed that quantitative amounts of Ca weight % and P weight % is statistically greater for all the three groups except the demineralized group. CESP with higher calcium content can remineralise enamel surface lesion.