Aerobic exercise inhibits renal EMT by promoting irisin expression in SHR

To determine the effect of aerobic exercise in different intensities on renal injury and epithelial-mesenchymal transformation (EMT) in the kidney of spontaneously hypertensive rats (SHR) and explore possible mechanisms, we subjected SHR to different levels of 14-week aerobic treadmill training. We tested the effects of aerobic exercise on irisin level, renal function, and EMT modulators in the kidney. We also treated angiotensin II-induced HK-2 cells with irisin and tested the changes in EMT levels. The data showed low and moderate aerobic exercise improved renal function and inhibited EMT through promoting irisin expression in SHR. However, high-intensity exercise training had no effect on renal injury and EMT in SHR but did significantly activate STAT3 phosphorylation in the kidney. These results clarify the mechanisms of exercise in improving hypertension-related renal injury and suggest that irisin might be a therapeutic target for patients with kidney injury. [Display omitted] •This study explored the effects of exercise intensity on nephropathy in hypertensive rats•Exercise improved renal function and inhibited EMT, but not at high intensity•The mechanism involved upregulation of irisin and its precursor FNDC5•Different effects of vigorous exercise may be because of irisin activation of STAT3 Biological sciences; Physiology; Molecular biology