Physical properties of food or bile redirection do not contribute to the intestinal adaptations after Roux‐en‐Y Gastric Bypass in rats

Objective Metabolic and morphological adaptations of the intestine have been suggested to play a role in the various therapeutic benefits of Roux‐en‐Y Gastric Bypass (RYGB) surgery. However, the precise underlying mechanisms remain unclear. In this study, the effects of physical properties of ingested food and redirection of biliopancreatic secretions on intestinal remodeling were investigated in RYGB operated rats. Methods RYGB employing two different Roux Limb (RL) lengths was performed on high fat diet induced obese rats. Post‐operatively, rats were fed either Solid or isocaloric Liquid diets. Metabolic and morphological remodeling of intestine was compared across both diet forms (Solid and Liquid diets) and surgical models (Short RL and Long RL). Results RYGB surgery in rats induced weight loss and improved glucose tolerance which was independent of physical properties of ingested food and biliopancreatic secretions. Intestinal glucose utilization after RYGB was not determined by either food form or biliopancreatic secretions. The GLUT‐1 expression in RL was not influenced by physical properties of food. Furthermore, both physical properties of food and biliopancreatic secretions showed no effects on intestinal morphological adaptations after RYGB. Conclusion Results of this study demonstrate that physical properties of food and bile redirection are not major determinants of intestinal remodeling after RYGB in rats. Metabolic and morphological adaptations of the intestine have been suggested to play a role in the various therapeutic benefits of Roux–en–Y Gastric Bypass (RYGB) surgery. However, the precise underlying mechanisms remain unclear. The results of our study show that RYGB surgery in rats induces weight loss and improves glucose tolerance, independent of physical properties of ingested food and biliopancreatic secretions. The regulation of intestinal glucose utilization after RYGB is not determined by food form or bile redirection. Furthermore, neither physical properties of food nor biliopancreatic secretions influence intestinal morphological adaptations in RYGB.