Generation of two PITX2 knock-out human induced pluripotent stem cell lines using CRISPR/Cas9 system

Generation of two PITX2 knock-out human induced pluripotent stem cell lines using CRISPR/Cas9 system

PITX2 is a homeobox gene located in the human 4q25 locus and is commonly associated with atrial fibrillation (AF). Here, we generated two PITX2 knock-out human induced pluripotent stem cell (iPSC) lines using CRISPR/Cas9 genome editing. The edited iPSCs maintained fullpluripotency, normal karyotype...

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Veröffentlicht in:Stem cell research 2022-12, Vol.65, p.102940-102940, Article 102940
Hauptverfasser: Mun, Dasom, Kang, Ji-Young, Chun, Yumin, Park, Da-Seul, Kim, Hyoeun, Yun, Nuri, Lee, Seung-Hyun, Joung, Boyoung
Format: Artikel
Sprache:eng
Schlagworte:
Atrial Fibrillation
CRISPR-Cas Systems - genetics
Humans
Induced Pluripotent Stem Cells
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Zusammenfassung:PITX2 is a homeobox gene located in the human 4q25 locus and is commonly associated with atrial fibrillation (AF). Here, we generated two PITX2 knock-out human induced pluripotent stem cell (iPSC) lines using CRISPR/Cas9 genome editing. The edited iPSCs maintained fullpluripotency, normal karyotype and spontaneousdifferentiation capability. This cell line provides a suitable model for investigating the physiopathologyof PITX2 mutation in atrial fibrillation.
ISSN:1873-5061
1876-7753
DOI:10.1016/j.scr.2022.102940