Cefepime and Amoxicillin Increase Metabolism and Enhance Caspofungin Tolerance of Candida albicans Biofilms

It is well known that prolonged antibiotic therapy alters the mucosal microbiota composition, increasing the risk of invasive fungal infection (IFI) in immunocompromised patients. The present study investigated the direct effect of β-lactam antibiotics cefepime (CEF) and amoxicillin (AMOX) on biofil...

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Veröffentlicht in:Frontiers in microbiology 2019-06, Vol.10, p.1337-1337
Hauptverfasser: Cordeiro, Rossana de Aguiar, Evangelista, Antonio Jose de Jesus, Serpa, Rosana, de Andrade, Ana Raquel Colares, Mendes, Patrícia Bruna Leite, de Oliveira, Jonathas Sales, de Alencar, Lucas Pereira, Pereira, Vandbergue Santos, Lima-Neto, Reginaldo Gonçalves, Brilhante, Raimunda Nogueira, Sidrim, José Júlio Costa, Maia, Débora Castelo Brancode Souza Collares, Rocha, Marcos Fábio Gadelha
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Sprache:eng
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Zusammenfassung:It is well known that prolonged antibiotic therapy alters the mucosal microbiota composition, increasing the risk of invasive fungal infection (IFI) in immunocompromised patients. The present study investigated the direct effect of β-lactam antibiotics cefepime (CEF) and amoxicillin (AMOX) on biofilm production by ATCC 10231. Antibacterials at the peak plasmatic concentration of each drug were tested against biofilms grown on polystyrene surfaces. Biofilms were evaluated for biomass production, metabolic activity, carbohydrate and protein contents, proteolytic activity, ultrastructure, and tolerance to antifungals. CEF and AMOX enhanced biofilm production by ATCC 10231, stimulating biomass production, metabolic activity, viable cell counts, and proteolytic activity, as well as increased biovolume and thickness of these structures. Nevertheless, AMOX induced more significant changes in biofilms than CEF. In addition, it was shown that AMOX increased the amount of chitin in these biofilms, making them more tolerant to caspofungin. Finally, it was seen that, in response to AMOX, biofilms produce Hsp70 - a protein with chaperone function related to stressful conditions. These results may have a direct impact on the pathophysiology of opportunistic IFIs in patients at risk.
ISSN:1664-302X
1664-302X
DOI:10.3389/fmicb.2019.01337