Borrelia miyamotoi infection in Apodemus spp. mice populating an urban habitat (Warsaw, Poland)

The two of three species of city-inhabiting (Warsaw, Poland) Apodemus spp. mice were showed to be infected with Borrelia miyamotoi, spirochete bacterium considered to be a tick-borne relapsing fever (TBRF) disease causative agent. The pathogen infection was ascertained based on bacterium DNA presence in a blood (obtained from the lateral tail vein) of the mice, using the nested polymerase chain reaction (PCR) technique. The B. miyamotoi carriers populated not only green spaces situated in the suburban areas but also recreational parks and lawns in the city centre proper. Moreover, we found no significant differences in the infection rate depending on mouse species. The total prevalence of infection at the level of 5.6% suggests the potential role of urban-settled rodents in the spreading of the tick-borne zoonosis, which pose high risk to public health. [Display omitted] •First confirmation of Borrelia miyamotoi infection in urban rodents in Poland.•The pathogen was found to infect city-inhabiting mice with a total prevalence of 5.6%.•Degree of human pressure within habitat did not affect rodents' infection rate.•Tick-borne zoonosis spread by mice could pose potential high risk to public health.