Prevalence of Rotavirus in shellfish from Southern Kerala

Aim: To study the prevalence of Rotavirus in shellfish from Southern Kerala. Materials and Methods: The shellfish samples after processing was concentrated using proteinase K. RNA was isolated from the concentrated samples using phenol chloroform method. Rota viral RNA was detected using reverse tra...

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Veröffentlicht in:Veterinary World 2014-10, Vol.7 (10), p.821-824
Hauptverfasser: Mohan, Vysakh, Rawat, Shriya, Lokesh, K.M, Mohan, H.V, Reddy, D. Avinash, Kumar, Ashok, Bhilegaonkar, K.N
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Sprache:eng
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Zusammenfassung:Aim: To study the prevalence of Rotavirus in shellfish from Southern Kerala. Materials and Methods: The shellfish samples after processing was concentrated using proteinase K. RNA was isolated from the concentrated samples using phenol chloroform method. Rota viral RNA was detected using reverse transcription-polymerase chain reaction. Results: A low prevalence of 2.5% (5/200) was obtained during the study. Rotavirus was detected in 2 out of 60 brown mussels (3.33%), 2 out of 70 yellow clams (2.86%) and 1 out of 70 black clams (1.43%). Conclusion: Low prevalence of Rotavirus was obtained in our study. A more extensive study need to be conducted to estimate the prevalence of enteric virus in shellfish. Keywords: concentration, proteinase K, reverse transcription-polymerase chain reaction, Rotavirus, shellfish.
ISSN:0972-8988
2231-0916
DOI:10.14202/vetworld.2014.821-824