Characterization of a human induced Pluripotent Stem (iPS) cell line (INCABRi002-A) derived from a primary myelofibrosis patient harboring the 5-bp insertion in CALR and the p.W146X mutation in TP53

Primary myelofibrosis (PMF) is a hematological malignancy characterized by activation of the JAK/STAT pathway and risk of leukemic transformation. In this study, we generated an induced Pluripotent Stem (iPS) cell line derived from a 65-year old male PMF patient carrying the 5-pb insertion in the CALR gene (CALRins5) and the c.437 G > A mutation in the TP53 gene (p.W146X). The newly derived PMF3.17 iPS cell line harbors the original mutations and was characterized as bona fide iPS. Resource tableUnlabelled TableUnique stem cell line identifierINCABRi002-AAlternative name(s) of stem cell linePMF3.17InstitutionBrazilian National Cancer Institute and D'Or Institute for Research and EducationContact information of distributorMartin Bonamino, PhD, mbonamino@inca.gov.br, Bárbara Monte Mór, PhD, barbara.montemor@inca.gov.br, Stevens Rehen, PhD, srehen@lance-ufrj.orgType of cell lineiPS cellOriginHumanAdditional origin infoAge: 65Sex: maleCell sourcePeripheral bloodClonalityClonalMethod of reprogrammingSendai VirusGenetic modificationNoType of modificationNot applicableAssociated diseasePrimary myelofibrosisGeneCALR gene: CALRins5, c.1154_1155insTTGTC, p.K385 fs*47TP53 gene: c.437 G > A, p.W146XMethod of modificationNot applicableName of transgene or resistanceNot applicableInducible/constitutive systemNot applicableDate archived/stock dateJuly 7th, 2015Cell line repository/bankNot applicableEthical approvalEthics Committee of the Brazilian National Cancer Institute (INCA) under the number 062/08. Ethics Review Board-competent authority obtained.