Network analysis and experimental verification of tanshinone ⅡA in treatment of autoimmune hepatitis

Objective To analyze the relationship between potential targets and signaling pathways of tanshinone Ⅱ A (Tan Ⅱ A) in the treatment of autoimmune hepatitis (AIH) based on network pharmacological analysis, and verify the mechanism of Tan Ⅱ A on AIH by animal experiments. Methods The relevant gene targets of Tan ⅡA and AIH were searched and screened through multiple databases, and the intersection genes between the drug and the disease were selected. Protein-protein interaction (PPI) network was subsequently constructed with the help of String platform, and the intersection genes were enriched and analyzed by Gene Ontology (GO) enrichment analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis. Then, the signaling pathway with the highest number of enriched genes was verified through animal experiments. Eighteen C57BL/6 mice were equally and randomly divided into normal, model and Tan ⅡA treatment groups. The contents of serum alanine aminotransferase (ALT) and aspartate aminotransferase (A