Evaluation of resazurin-based assay for rapid detection of polymyxin-resistant gram-negative bacteria

Colistin resistance is considered a serious problem due to a lack of alternative antibiotics. The Rapid ResaPolymyxin Acinetobacter/Pseudomonas NP test is a resazurin reduction-based technique that relies on the visual detection of bacterial growth in the presence of a defined concentration of colis...

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Veröffentlicht in:	BMC microbiology 2020-01, Vol.20 (1), p.7-7, Article 7
Hauptverfasser:	Jia, Huaiyu, Fang, Renchi, Lin, Jie, Tian, Xuebin, Zhao, Yajie, Chen, Lijiang, Cao, Jianming, Zhou, Tieli
Format:	Artikel
Sprache:	eng
Schlagworte:	Acinetobacter Acinetobacter baumannii - isolation & purification Antibiotics Bacteria Clinical isolates Clinical medicine Colistin Colistin-resistant Diagnostic Tests, Routine Drug resistance Drug Resistance, Bacterial E coli Evaluation Gram-negative bacteria Gram-Negative Bacteria - isolation & purification Growth Hospitals, Teaching Klebsiella Klebsiella pneumoniae Klebsiella pneumoniae - isolation & purification Laboratories Methods Microbial drug resistance Microbial Sensitivity Tests Microbiological assay Observations Oxazines - chemistry Performance evaluation Polymyxins - pharmacology Pseudomonas Pseudomonas aeruginosa Pseudomonas aeruginosa - isolation & purification Rapid diagnosis Rapid ResaPolymyxin Acinetobacter/Pseudomonas NP test Sensitivity and Specificity Testing Xanthenes - chemistry
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Zusammenfassung:	Colistin resistance is considered a serious problem due to a lack of alternative antibiotics. The Rapid ResaPolymyxin Acinetobacter/Pseudomonas NP test is a resazurin reduction-based technique that relies on the visual detection of bacterial growth in the presence of a defined concentration of colistin. The aim of this study was to evaluate the performance of the Rapid ResaPolymyxin Acinetobacter/Pseudomonas NP test in the detection of colistin susceptibility in common clinical Gram-negative bacteria. A total of 253 clinical isolates from a teaching hospital, including Acinetobacter baumanii (n = 58, 8 colistin-resistant), Pseudomonas aeruginosa (n = 61, 11 colistin-resistant), Klebsiella pneumoniae (n = 70, 20 colistin-resistant) and Escherichia coli (n = 64, 14 colistin-resistant) were tested in this study. The sensitivity and specificity of the Rapid ResaPolymyxin Acinetobacter/Pseudomonas NP test compared to Broth microdilution method was 100 and 99%, respectively. Our results suggest that Rapid ResaPolymyxin Acinetobacter/Pseudomonas NP test could be used as an accurate detection method for colistin resistance.
ISSN:	1471-2180 1471-2180
DOI:	10.1186/s12866-019-1692-3