Association of BRAF V600E Mutant Allele Proportion with the Dissemination Stage of Papillary Thyroid Cancer

The early identification of aggressive forms of cancer is of high importance in treating papillary thyroid cancer (PTC). Disease dissemination is a major factor influencing patient survival. Mutation status of oncogene, V600E, is proposed to be an indicator of disease recurrence; however, its influe...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Biomedicines 2024-02, Vol.12 (3), p.477
Hauptverfasser: Blazekovic, Ivan, Samija, Ivan, Perisa, Josipa, Gall Troselj, Koraljka, Regovic Dzombeta, Tihana, Radulovic, Petra, Romic, Matija, Granic, Roko, Sisko Markos, Ines, Frobe, Ana, Kusic, Zvonko, Jukic, Tomislav
Format: Artikel
Sprache:eng
Schlagworte:
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:The early identification of aggressive forms of cancer is of high importance in treating papillary thyroid cancer (PTC). Disease dissemination is a major factor influencing patient survival. Mutation status of oncogene, V600E, is proposed to be an indicator of disease recurrence; however, its influence on PTC dissemination has not been deciphered. This study aimed to explore the association of the frequency of V600E alleles in PTC with disease dissemination. In this study, 173 PTC samples were analyzed, measuring the proportion of V600E alleles by qPCR, which was then normalized against the proportion of tumor cells. Semiquantitative analysis of BRAF V600E mutant protein was performed by immunohistochemistry. The V600E mutation was present in 60% of samples, while the normalized frequency of mutated alleles ranged from 1.55% to 92.06%. There was no significant association between the presence and/or proportion of the V600E mutation with the degree of PTC dissemination. However, the presence of the mutation was significantly linked with angioinvasion. This study's results suggest that there is a heterogeneous distribution of the mutation and the presence of oligoclonal forms of PTC. It is likely that the mutation alone does not significantly contribute to PTC aggressiveness.
ISSN:2227-9059
2227-9059
DOI:10.3390/biomedicines12030477