mmunohistochemical Identification And Quantification Of Brown Adipocytes In Human Adipose Tissue

Background: Obesity is a worldwide challenge and is closely connected to many metabolic diseases. Two types of adipose tissue, white adipose tissue (WAT) and brown adipose tissue (BAT) have been identified. White fat cells store chemical energy, brown adipocytes defend against hypothermia, obesity and diabetes. Objective: To localize and quantify brown adipocytes in human subcutaneous (S) and visceral (V) adipose tissue by histology and immunohistochemistry. Type of the study: A cross –sectional study. Methods: Adipose tissue was obtained from histopathology specimens taken from ten patients, of different age, sex and body mass index (BMI), undergoing surgery for different pathologies. Immunohistochemistry for detection of UCP (Uncoupling Protein) in S and V adipose tissue depots was performed, and percentage of the positive pixels for UCP color intensity was measured and statistical analysis performed.data was expressed as mean ± standard deviation (SD) and analyzed using student t-test to compare values