Optimization of a Method for the Simultaneous Extraction of Polar and Non-Polar Oxylipin Metabolites, DNA, RNA, Small RNA, and Protein from a Single Small Tissue Sample

A more comprehensive picture of tissue biology can be obtained through the application and integration of multiple omic technologies. However, the common challenge in working with a precious sample is having a sample too small to separately extract analytes of interest for each experiment. Consideri...

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Veröffentlicht in:Methods and protocols 2020-08, Vol.3 (3), p.61
Hauptverfasser: Hasegawa, Yu, Otoki, Yurika, McClorry, Shannon, Coates, Laurynne C., Lombardi, Rachel L., Taha, Ameer Y., Slupsky, Carolyn M.
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Sprache:eng
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Zusammenfassung:A more comprehensive picture of tissue biology can be obtained through the application and integration of multiple omic technologies. However, the common challenge in working with a precious sample is having a sample too small to separately extract analytes of interest for each experiment. Considering the high heterogeneity that can be present in a single tissue sample, extracting all biomolecules from a single and undivided tissue is preferable because it allows direct comparison of results. Here, we combined a modified Folch extraction method with DNA, RNA, small RNA, and protein extraction using two commercial kits, which allowed us to extract polar metabolites and non-polar oxylipin metabolites, DNA, RNA, small RNA, and protein simultaneously from a small tissue sample. The method was validated in terms of quantity and quality of analytes for downstream analyses.
ISSN:2409-9279
2409-9279
DOI:10.3390/mps3030061