Frequency of transovarial transmission of dengue virus in Aedes aegypti mosquitoes in endemic areas of dengue hemorrhagic fever in Ceper District, Klaten Regency, Central Java Province, Indonesia

Klaten Regency is an area in Central Java Province with a high rate of endemic dengue hemorrhagic fever. This study aims to demonstrate a correlation between transovarial transmission and the incidence of dengue haemorrhagic fever in Ceper District, Klaten Regency. Aedes larvae and pupae were collected from January 2019 to March 2019 from various containers, using gamadotic and dipper tools, in Ngawonggo, Kajen, and Meger villages, which have high, medium, and low numbers of dengue cases, respectively. The larvae and pupae obtained were reared into adults. The presence of dengue virus in head squashes of 1-week-old female Ae. aegypti was identified based on an immunocytochemical assay using DSSE10 monoclonal antibody, made at Universitas Gadjah Mada as a primary antibody. The frequency of transovarial transmission of dengue virus (TIR) in Ae. aegypti in the three villages was 35.7%. The highest frequency occurred in Ngawonggo Village, at 40 %, and the lowest in Meger Village, at 28%, while the frequency in Kajen Village was 36.9 %. This study proves that there is a strong correlation (r = 0.99664) between transovarial transmission and the incidence of dengue hemorrhagic fever in Ceper District, Klaten Regency, Central Java.