The boosting effects of melatonin on the expression of related genes to oocyte maturation and antioxidant pathways: a polycystic ovary syndrome- mouse model

Melatonin, as a free radical scavenger exhibiting genomic actions, regulates the antioxidant genes expression and apoptosis mechanisms. In polycystic ovary syndrome (PCOS) patients, an imbalance between free radicals and antioxidants in follicular fluid leads to oxidative stress, aberrant folliculog...

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Veröffentlicht in:	Journal of ovarian research 2022-01, Vol.15 (1), p.11-11, Article 11
Hauptverfasser:	Nikmard, Fatemeh, Hosseini, Elham, Bakhtiyari, Mehrdad, Ashrafi, Mahnaz, Amidi, Fardin, Aflatoonian, Reza
Format:	Artikel
Sprache:	eng
Schlagworte:	Analysis Animals Antioxidant Antioxidants Antioxidants - metabolism Apoptosis Apoptosis - drug effects Apoptosis - genetics BAX gene bcl-2-Associated X Protein - genetics Binding sites Bone Morphogenetic Protein 15 - genetics Data analysis Dehydroepiandrosterone - toxicity Disease Models, Animal Female Follicular fluid Folliculogenesis Free radicals Free radicals (Chemistry) Gene expression Genes Glutathione Peroxidase - genetics Growth Differentiation Factor 9 - genetics In Vitro Oocyte Maturation Techniques In-vitro maturation Melatonin Melatonin - pharmacology Mice Oocytes Oocytes - drug effects Oocytes - metabolism Oogenesis - drug effects Oogenesis - genetics Ovaries Ovulation Oxidative stress PCOS Polycystic ovary syndrome Polycystic Ovary Syndrome - chemically induced Polycystic Ovary Syndrome - genetics Polycystic Ovary Syndrome - metabolism Polycystic Ovary Syndrome - pathology Proto-Oncogene Proteins c-bcl-2 - genetics Reactive Oxygen Species - metabolism Stein-Leventhal syndrome Superoxide dismutase Superoxide Dismutase-1 - genetics
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Zusammenfassung:	Melatonin, as a free radical scavenger exhibiting genomic actions, regulates the antioxidant genes expression and apoptosis mechanisms. In polycystic ovary syndrome (PCOS) patients, an imbalance between free radicals and antioxidants in follicular fluid leads to oxidative stress, aberrant folliculogenesis, and intrinsic defects in PCOS oocytes. In this experimental mouse model study, oocytes of PCOS and the control groups were cultured in different melatonin concentrations (10 , 10 , and 10 M) to investigate the expression of oocyte maturation-related genes (Gdf9/Bmp15), antioxidant-related genes (Gpx1/Sod1), apoptotic biomarkers (Bcl2/Bax) and total intracellular ROS levels. Gdf9 and Bmp15, Gpx1 and Sod1 were up-regulated in PCOS and control oocytes cultured in all melatonin concentrations compared to those cultured in IVM basal medium (P
ISSN:	1757-2215 1757-2215
DOI:	10.1186/s13048-022-00946-w