A simple method for the separation of triacylglycerols from fatty acids released in lipase assays

A simple method for the separation of triacylglycerols from fatty acids released in lipase assays

A simple procedure for the partition of triacylglycerols from albumin-bound fatty acids is described. This procedure is based on the ability of fumed silicon dioxide to remove emulsified triacylglycerols from aqueous media. The method was developed for the assay of lipoprotein lipase activity but it...

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Veröffentlicht in:Journal of lipid research 1988-11, Vol.29 (11), p.1549-1552
Hauptverfasser: Borensztajn, J, Reddy, M N, Gladstone, A R
Format: Artikel
Sprache:eng
Schlagworte:
Albumins - isolation & purification
Animals
Fatty Acids - analysis
Hydrolysis
Lipase
Milk - enzymology
Triglycerides - isolation & purification
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Zusammenfassung:A simple procedure for the partition of triacylglycerols from albumin-bound fatty acids is described. This procedure is based on the ability of fumed silicon dioxide to remove emulsified triacylglycerols from aqueous media. The method was developed for the assay of lipoprotein lipase activity but it may be used for the assay of other lipases.
ISSN:0022-2275
DOI:10.1016/S0022-2275(20)38432-7