Antagonistic activity of two Bacillus strains against Fusarium oxysporum f. sp. capsici ( FOC -1) causing Fusarium wilt and growth promotion activity of chili plant

f. sp. (Foc) poses a significant position in agriculture that has a negative impact on chili plant in terms of growth, fruit quality, and yield. Biological control is one of the promising strategies to control this pathogen in crops. Chili is considered as one of the most important crops in the Hyderabad region that is affected by Fusarium wilt disease. The pathogen was isolated from the infected samples in the region and was confirmed by morphological characteristics and PCR with a band of 488 bp. The bacterial strains were isolated from the rhizosphere soil of healthy plant and also confirmed by PCR with a band of 1,542 bp.The molecular characterization of the fungal and bacterial strain has shown 99.9% homology with the retrieved sequences of f. sp. and from NCBI. The 1-month-old Ghotki chili plants were inoculated with 1×10 cfu spore/ml suspension and confirmed that the -1 is responsible for chili Fusarium wilt disease. Subsequently, among the 33 screened strains, only 11 showed antagonistic activity against . Out of these, only two strains (AM13 and AM21) have shown maximum antagonistic activity against the pathogen by reducing the infection and promoting growth parameters of chili plants under both and greenhouse conditions. The study suggested that biological control is the most promising control strategy for the management of Fusarium wilt of chili in the field.