A comparison of the antileukaemic effects of recombinant human tumour necrosis factor-α and its muteins on leukaemia L1210 and leukaemia P388 in mice

WE investigated the influence of recombinant human tumour necrosis factor alpha (TNF-α) and its derivatives termed muteins III, V, VI－in which the first 3 to 7 amino acids of native TNF-α have been replaced---on the survival time of mice inoculated with leukaemia L1210 or leukaemia P338. TNF-tx prolonged the survival of mice with leukaemia L1210 but did not have any therapeutic activity in leukaemia P388-bearing mice. Muteins-treated mice with leukaemia P388 Hved longer than animals receiving TNF-α, while those inoculated with leukaemia L1210 did not show any significant prolongation of life compared with the TNF-α treated group. The results presented in this report indicate that the antileukaemic activity of TNF-α is governed at least in part by the nature of the N-terminal amino acids.