The Immune Atlas of Human Deciduas With Unexplained Recurrent Pregnancy Loss

Recurrent pregnancy loss (RPL) is a common fertility problem that affects 1%-2% of couples all over the world. Despite exciting discoveries regarding the important roles of the decidual natural killer cell (dNK) and regulatory T cell in pregnancy, the immune heterogeneity in patients with unexplaine...

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Veröffentlicht in:Frontiers in immunology 2021-06, Vol.12, p.689019-689019
Hauptverfasser: Chen, Pengfei, Zhou, Liying, Chen, Jiying, Lu, Ying, Cao, Chaoxia, Lv, Shuangli, Wei, Zhihong, Wang, Liping, Chen, Jiao, Hu, Xinglin, Wu, Zijing, Zhou, Xiaohua, Su, Danna, Deng, Xuefeng, Zeng, Changchun, Wang, Huiyun, Pu, Zuhui, Diao, Ruiying, Mou, Lisha
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Sprache:eng
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Zusammenfassung:Recurrent pregnancy loss (RPL) is a common fertility problem that affects 1%-2% of couples all over the world. Despite exciting discoveries regarding the important roles of the decidual natural killer cell (dNK) and regulatory T cell in pregnancy, the immune heterogeneity in patients with unexplained recurrent pregnancy loss (URPL) remains elusive. Here, we profiled the transcriptomes of 13,953 CD45 + cells from three normal and three URPL deciduas. Based on our data, the cellular composition revealed three major populations of immune cells including dNK cell, T cell, and macrophage, and four minor populations including monocytes, dendritic cell (DC), mast cell, and B cell. Especially, we identified a subpopulation of CSF1+ CD59+ KIRs-expressing dNK cells in normal deciduas, while the proportion of this subpopulation was decreased in URPL deciduas. We also identified a small subpopulation of activated dDCs that were accumulated mainly in URPL deciduas. Furthermore, our data revealed that in decidua at early pregnancy, CD8 + T cells exhibited cytotoxic properties. The decidual macrophages expressed high levels of both M1 and M2 feature genes, which made them unique to the conventional M1/M2 classification. Our single-cell data revealed the immune heterogeneity in decidua and the potentially pathogenic immune variations in URPL.
ISSN:1664-3224
1664-3224
DOI:10.3389/fimmu.2021.689019