Corynebacterium diphtheriae putative tellurite-resistance protein (CDCE8392_0813) contributes to the intracellular survival in human epithelial cells and lethality of Caenorhabditis elegans
Corynebacterium diphtheriae , the aetiologic agent of diphtheria, also represents a global medical challenge because of the existence of invasive strains as causative agents of systemic infections. Although tellurite (TeO32−) is toxic to most microorganisms, TeO32−-resistant bacteria, including C. d...
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Veröffentlicht in: | Memórias do Instituto Oswaldo Cruz 2015-08, Vol.110 (5), p.662-668 |
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Sprache: | eng |
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Zusammenfassung: | Corynebacterium diphtheriae , the aetiologic agent of diphtheria, also
represents a global medical challenge because of the existence of
invasive strains as causative agents of systemic infections. Although
tellurite (TeO32−) is toxic to most microorganisms,
TeO32−-resistant bacteria, including C. diphtheriae, exist in
nature. The presence of TeO32−-resistance (TeR) determinants in
pathogenic bacteria might provide selective advantages in the natural
environment. In the present study, we investigated the role of the
putative TeR determinant (CDCE8392_813 gene) in the virulence
attributes of diphtheria bacilli. The disruption of CDCE8392_0813 gene
expression in the LDCIC_L1 mutant increased susceptibility to
TeO32− and reactive oxygen species (hydrogen peroxide), but not
to other antimicrobial agents. The LDCIC-L1 mutant also showed a
decrease in both the lethality of Caenorhabditis elegans and the
survival inside of human epithelial cells compared to wild-type strain.
Conversely, the haemagglutinating activity and adherence to and
formation of biofilms on different abiotic surfaces were not regulated
through the CDCE8392_0813 gene. In conclusion, the CDCE8392_813 gene
contributes to the TeR and pathogenic potential of C. diphtheriae. |
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ISSN: | 1678-8060 0074-0276 1678-8060 |
DOI: | 10.1590/0074-02760140479 |