In Vitro Maturation of Viable Islets from Partially Digested Young Pig Pancreas

Isolation of islets from market-sized pigs is costly, with considerable islet losses from fragmentation occurring during isolation and tissue culture. Fetal and neonatal pigs yield insulin unresponsive islet-like cell clusters that become glucose-responsive after extended periods of time. Both issue...

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Veröffentlicht in:	Cell transplantation 2014-04, Vol.23 (3), p.263-272
Hauptverfasser:	Lamb, Morgan, Laugenour, Kelly, Liang, Ouwen, Alexander, Michael, Foster, Clarence E., Lakey, Jonathan R. T.
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Apoptosis Cell Separation - economics Cell Separation - methods Cell Survival Collagenases - metabolism Glucose - metabolism Islets of Langerhans - cytology Islets of Langerhans - metabolism Islets of Langerhans Transplantation Male Pancreas - cytology Pancreas - metabolism Swine
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Beschreibung
Zusammenfassung:	Isolation of islets from market-sized pigs is costly, with considerable islet losses from fragmentation occurring during isolation and tissue culture. Fetal and neonatal pigs yield insulin unresponsive islet-like cell clusters that become glucose-responsive after extended periods of time. Both issues impact clinical applicability and commercial scale-up. We have focused our efforts on a cost-effective scalable method of isolating viable insulin-responsive islets. Young Yorkshire pigs (mean age 20 days, range 4–30 days) underwent rapid pancreatectomy (
ISSN:	0963-6897 1555-3892
DOI:	10.3727/096368912X662372