VPS35 Deficiency or Mutation Causes Dopaminergic Neuronal Loss by Impairing Mitochondrial Fusion and Function

Vacuolar protein sorting-35 (VPS35) is a retromer component for endosomal trafficking. Mutations of VPS35 have been linked to familial Parkinson’s disease (PD). Here, we show that specific deletion of the VPS35 gene in dopamine (DA) neurons resulted in PD-like deficits, including loss of DA neurons and accumulation of α-synuclein. Intriguingly, mitochondria became fragmented and dysfunctional in VPS35-deficient DA neurons, phenotypes that could be restored by expressing VPS35 wild-type, but not PD-linked mutant. Concomitantly, VPS35 deficiency or mutation increased mitochondrial E3 ubiquitin ligase 1 (MUL1) and, thus, led to mitofusin 2 (MFN2) degradation and mitochondrial fragmentation. Suppression of MUL1 expression ameliorated MFN2 reduction and DA neuron loss but not α-synuclein accumulation. These results provide a cellular mechanism for VPS35 dysfunction in mitochondrial impairment and PD pathogenesis. [Display omitted] •Genetic deletion of VPS35 in dopamine neurons results in PD-relevant pathology•VPS35 deficiency or mutation impairs mitochondrial fusion and function•VPS35 deficiency or mutation increases MUL1-mediated MFN2 degradation•Mul1 suppression in DA neurons ameliorates VPS35 deficiency-induced neuronal loss Tang et al. find that VPS35 in mouse dopaminergic neurons is required for preventing dopaminergic neuronal loss. The function of VPS35 on dopamine neuronal survival is likely due to VPS35 promotion of MUL1 degradation, thus increasing MFN2 protein stability and mitochondrial fusion.