Mobile and Self‐Sustained Data Storage in an Extremophile Genomic DNA

DNA has been pursued as a novel biomaterial for digital data storage. While large‐scale data storage and random access have been achieved in DNA oligonucleotide pools, repeated data accessing requires constant data replenishment, and these implementations are confined in professional facilities. Here, a mobile data storage system in the genome of the extremophile Halomonas bluephagenesis, which enables dual‐mode storage, dynamic data maintenance, rapid readout, and robust recovery. The system relies on two key components: A versatile genetic toolbox for the integration of 10–100 kb scale synthetic DNA into H. bluephagenesis genome and an efficient error correction coding scheme targeting noisy nanopore sequencing reads. The storage and repeated retrieval of 5 KB data under non‐laboratory conditions are demonstrated. The work highlights the potential of DNA data storage in domestic and field scenarios, and expands its application domain from archival data to frequently accessed data. In this study, a data storage system in Halomonas bluephagenesis genomes, which supports frequent and rapid data retrieval and automated data regeneration in non‐professional environments, are developed. This system expands the application scenarios of DNA data storage to frequent “warm data” accessing in household and field settings, and also underscores the potentials of living cells and long‐fragment DNA for data storage.