Immunotherapeutic Effects of Different Doses of Mycobacterium tuberculosis ag85a/b DNA Vaccine Delivered by Electroporation

Tuberculosis (TB) is a major global public health problem. New treatment methods on TB are urgently demanded. Ninety-six female BALB/c mice were challenged with 2×10 colony-forming units (CFUs) of MTB H Rv through tail vein injection, then was treated with 10μg, 50μg, 100μg, and 200μg of (MTB) chimeric DNA vaccine delivered by intramuscular injection (IM) and electroporation (EP), respectively. The immunotherapeutic effects were evaluated immunologically, bacteriologically, and pathologically. Compared with the phosphate-buffered saline (PBS) group, the CD4 IFN-γ T cells% in whole blood from 200 µg DNA IM group and four DNA EP groups increased significantly (