Synergistic Generation of Reactive Oxygen Species by Visible Light Activated TiO2 and S. Enterica Interaction

A lot of human activities have negative impact on water quality and sometimes result in the biological water contamination. Currently used chemical (chlorine, ozone, and etc.) and physical (UV) water disinfection methods have strong environmental disadvantages or suffers from limited efficiency. To overcome these problems, scientists suggest to use photocatalyst activated advanced oxidation processes. One of the most studied photocatalysts which attracts a lot of research interest is titanium dioxide. TiO application for the disinfection of water, air or surfaces is increasingly encouraged by researchers. However, to unlock its full potential it is highly desirable to make it suitable for the visible light activation. In the current study the effect of visible light assisted photocatalytic treatment to the outer membrane permeability of bacteria and how it changes under different titanium dioxide concentrations was analysed. The results from the treatment of relatively complex bacteria organism were compared to the visible light activated TiO ability to oxidise considerably simpler objects like methylene blue molecules. The efficiency of TiO photocatalytic disinfection process was evaluated using spread plate technique. Membrane permeability of the treated bacteria was determined by NPN uptake factor assay. Generation of intracellular reactive oxygen species was evaluated by Dichlorodihydrofluorescein diacetate fluorescence measurements. The key finding of this study was that intense wide spectrum visible light irradiation and TiO powder synergistically inactivate bacteria and halt its potential to form colonies. High amounts of intracellular reactive oxygen species could be seen as the main suspects for the observed inactivation of