Chromosomal Evolution and Evolutionary Relationships of Lebiasina Species (Characiformes, Lebiasinidae)
We present the first cytogenetic data for and with the aim of (1) investigating evolutionary events within and their relationships with other Lebiasinidae genera and (2) checking the evolutionary relationships between Lebiasinidae and Ctenoluciidae. Both species have a diploid number 2n = 36 with si...
Gespeichert in:
Veröffentlicht in: | International journal of molecular sciences 2019-06, Vol.20 (12), p.2944 |
---|---|
Hauptverfasser: | , , , , , , , , , , |
Format: | Artikel |
Sprache: | eng |
Schlagworte: | |
Online-Zugang: | Volltext |
Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
Zusammenfassung: | We present the first cytogenetic data for
and
with the aim of (1) investigating evolutionary events within
and their relationships with other Lebiasinidae genera and (2) checking the evolutionary relationships between Lebiasinidae and Ctenoluciidae. Both species have a diploid number 2n = 36 with similar karyotypes and microsatellite distribution patterns but present contrasting C-positive heterochromatin and CMA
banding patterns. The remarkable interstitial series of C-positive heterochromatin occurring in
is absent in
. Accordingly,
shows the ribosomal DNA sites as the only GC-rich (CMA
) regions, while
shows evidence of a clear intercalated CMA
banding pattern. In addition, the multiple 5S and 18S rDNA sites in
contrast with single sites present in
. Comparative genomic hybridization (CGH) experiments also revealed a high level of genomic differentiation between both species. A polymorphic state of a conspicuous C-positive, CMA
, and (CGG)n band was found only to occur in
females, and its possible relationship with a nascent sex chromosome system is discussed. Whole chromosome painting (WCP) and CGH experiments indicate that the
species examined and
share similar chromosomal sequences, thus supporting the relatedness between them and the evolutionary relationships between the Lebiasinidae and Ctenoluciidae families. |
---|---|
ISSN: | 1422-0067 1661-6596 1422-0067 |
DOI: | 10.3390/ijms20122944 |