Nuclease activity of Plasmodium falciparum Alba family protein PfAlba3

Plasmodium falciparum Alba domain-containing protein Alba3 (PfAlba3) is ubiquitously expressed in intra-erythrocytic stages of Plasmodium falciparum, but the function of this protein is not yet established. Here, we report an apurinic/apyrimidinic site-driven intrinsic nuclease activity of PfAlba3 assisted by divalent metal ions. Surface plasmon resonance and atomic force microscopy confirm sequence non-specific DNA binding by PfAlba3. Upon binding, PfAlba3 cleaves double-stranded DNA (dsDNA) hydrolytically. Mutational studies coupled with mass spectrometric analysis indicate that K23 is the essential residue in modulating the binding to DNA through acetylation-deacetylation. We further demonstrate that PfSir2a interacts and deacetylates K23-acetylated PfAlba3 in favoring DNA binding. Hence, K23 serves as a putative molecular switch regulating the nuclease activity of PfAlba3. Thus, the nuclease activity of PfAlba3, along with its apurinic/apyrimidinic (AP) endonuclease feature identified in this study, indicates a role of PfAlba3 in DNA-damage response that may have a far-reaching consequence in Plasmodium pathogenicity. [Display omitted] •PfAlba3 exhibits abasic site-driven endonuclease activity•DNA binding by PfAlba3 is sequence non-specific and cooperative•Divalent metal ions assist hydrolytic cleavage of DNA by PfAlba3•PfAlba3 endonuclease activity is modulated by acetylation-deacetylation of K23 Banerjee et al. show that PfAlba3 exhibits apurinic/apyrimidinic site-driven endonuclease activity regulated by acetylation and deacetylation of K23.