Polyvinyl alcohol-glutaraldehyde as solid-phase in ELISA for schistosomiasis

Polyvinyl alcohol-glutaraldehyde as solid-phase in ELISA for schistosomiasis

Soluble adult Schistosoma mansoni antigen preparation (SWAP) was covalently fixed onto polyvinyl alcohol-glutaraldehyde discs and an enzyme linked-immunosorbent assay (ELISA) was set up. The best conditions for the assay were established and it was found that small amount of antigen such as 1.5 micr...

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Veröffentlicht in:Revista do Instituto de Medicina Tropical de São Paulo 1997-05, Vol.39 (3), p.155-158
Hauptverfasser: Araújo, A M, Barbosa, G H, Diniz, J R, Malagueño, E, Azevedo, W M, de Carvalho Júnior, L B
Format: Artikel
Sprache:eng
Schlagworte:
Animals
ELISA
Enzyme-Linked Immunosorbent Assay
Glutaral
Glutaraldehyde
Humans
Polyvinyl Alcohol
Rabbits
Schistosoma manson
Schistosomiasis - diagnosis
TROPICAL MEDICINE
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Beschreibung
Zusammenfassung:Soluble adult Schistosoma mansoni antigen preparation (SWAP) was covalently fixed onto polyvinyl alcohol-glutaraldehyde discs and an enzyme linked-immunosorbent assay (ELISA) was set up. The best conditions for the assay were established and it was found that small amount of antigen such as 1.5 micrograms was required. A comparison between this procedure and the conventional ELISA was proceeded. A reliable method of antigen immobilization was achieved and the low prices of the employed reagents are economically attractive.
ISSN:0036-4665
1678-9946
0036-4665
1678-9946
DOI:10.1590/S0036-46651997000300006