Bacterial host adaptation through sequence and structural variations of a single type III effector gene

Molecular mechanisms underlying quantitative variations of pathogenicity remain elusive. Here, we identified the Xanthomonas campestris XopJ6 effector that triggers disease resistance in cauliflower and Arabidopsis thaliana. XopJ6 is a close homolog of the Ralstonia pseudosolanacearum PopP2 YopJ family acetyltransferase. XopJ6 is recognized by the RRS1-R/RPS4 NLR pair that integrates a WRKY decoy domain mimicking effector targets. We identified a XopJ6 natural variant carrying a single residue substitution in XopJ6 WRKY-binding site that disrupts interaction with WRKY proteins. This mutation allows XopJ6 to evade immune perception while retaining some XopJ6 virulence functions. Interestingly, xopJ6 resides in a Tn3-family transposon likely contributing to xopJ6 copy number variation (CNV). Using synthetic biology, we demonstrate that xopJ6 CNV tunes pathogen virulence on Arabidopsis through gene dosage-mediated modulation of xopJ6 expression. Together, our findings highlight how sequence and structural genetic variations restricted at a particular effector gene contribute to bacterial host adaptation. [Display omitted] •Xanthomonas campestris XopJ6 effector protein is a YopJ family acetyltransferase•XopJ6 avirulence activity triggers immunity in Arabidopsis and cauliflower accessions•A XopJ6 natural variant evades recognition by the RRS1-R/RPS4 NLR pair•xopJ6 copy number variation modulates pathogen virulence on Arabidopsis Classification Description: Genetics; Bacteriology; Molecular plant pathology