Increased expressions of CD123, CD63, CD203c, and Fc epsilon receptor I on blood leukocytes of allergic asthma

Altered basophil identification markers have been discovered to associate with allergic asthma (AA) in recent years. However, little is known about the expression of basophil markers in blood granulocytes. To parallel test blood basophils in peripheral blood mononuclear cell (PBMC) and granulocyte populations of patients with AA and AA combined with allergic rhinitis (ARA) The expressions of surface molecules were determined flow cytometry. CD123 expressing cells in blood were isolated using a cell sorting technique, and mouse AA models were employed for study. The numbers of CD123 HLA-DR cells in the granulocytes of AA and ARA patients markedly increased. However, only 49.7% of CD123 HLA-DR cells in granulocytes and 99.0% of CD123 HLA-DR cells in PBMCs were basophils. Almost all CD123 HLA-DR cells expressed CD63 regardless in granulocytes or PBMC. The numbers of CD63, Fc epsilon receptor I (FcεRI), and CD203c expressing cells markedly enhanced in CD123 HLA-DR granulocytes of AA and ARA patients. Mean fluorescence intensity (MFI) of CD63 and CD203c expressions on CD123 HLA-DR PBMC and granulocytes of AA and ARA patients dramatically elevated. House dust mite extract (HDME) and wild allergen extract (ASWE) enhanced the numbers of CD63 CD123 HLA-DR granulocytes and PBMC and the MFI of CD203c expression on CD123 HLA-DR granulocyte of AA and ARA patients. Histamine, tryptase, and PGD2 enhanced proportions of CD123 KU812 cells. ASWE- and HDME-induced AA mice showed upregulated CD63 expression on basophils. In conclusion, upregulated expressions of CD123, CD203c, CD63, and FcεRIα in PBMC and granulocytes of patients with AA and ARA suggest that CD123 HLA-DR cells may contribute to the development of AA and ARA.