Protocol to assess extravasation of fluorescent molecules in mice after ultrasound-mediated blood-brain barrier opening

Blood-brain barrier disruption (BBBD) using focused ultrasound (FUS) and microbubbles (MBs) is an effective tool for therapeutic delivery to the brain. Here, we present an optimized protocol for quantifying fluorescent molecules extravasation in mice. We describe steps for ultrasound treatment, inje...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:STAR protocols 2024-03, Vol.5 (1), p.102770, Article 102770
Hauptverfasser: Peko, Lea, Katz, Sharon, Gattegno, Roni, Ilovitsh, Tali
Format: Artikel
Sprache:eng
Schlagworte:
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:Blood-brain barrier disruption (BBBD) using focused ultrasound (FUS) and microbubbles (MBs) is an effective tool for therapeutic delivery to the brain. Here, we present an optimized protocol for quantifying fluorescent molecules extravasation in mice. We describe steps for ultrasound treatment, injection of MBs and fluorescent dyes, brain harvesting, microscopy imaging, and image postprocessing algorithm. Our protocol has proven to successfully conduct a diameter-dependent analysis that measures vascular leakage following FUS-mediated BBBD at a single blood vessel resolution. For complete details on the use and execution of this protocol, please refer to Katz et al.1 [Display omitted] •Quantify BBB opening as a function of vessel diameter at single blood vessel resolution•High resolution of 1 μm for vessels with a diameter
ISSN:2666-1667
2666-1667
DOI:10.1016/j.xpro.2023.102770