The influence of various feed additives on the development of duodenal goblet cells in broilers

A comparative study was carried out on the effect of three drugs on the development of goblet cells in the duodenum of broilers. In the first experiment, day-old broilers Smena-8 were divided into 4 groups. The experimental groups received the phytobiotic Butitan with the main diet: E1-0.25%; E2-0.5% and E3-0.75%. In the second experiment, 3 groups were formed from broilers of the Konkurent cross; additives were added for the first 3 days. The Vetom group received a probiotic based on Bacillus subtilis (0.006%); Enterosgel group – Polymethylsiloxane polyhydrate sorbent (0.008%). Histological studies were carried out in the first experiment at 1, 7 and 42 days, in the second at 1, 4, 49 days. Sections were stained to identify acidic and neutral mucus. The number of goblet cells in the field of view was counted and their density over an area of 1000 μm2 was calculated. By the end of the first experiment, the density of neutral goblet cells in the villi E1 and E2 and crypts E2 and E3 increases by 17% and 7.5%, acidic goblet cells increase in the crypts by 32.6% and 33.1%. The probiotic increased the density of acidic goblet cells in the crypts (64%; P≤0.001) without affecting the villi. Enetrosorbent reduces the density of neutral and increases the density of acidic goblet cells in the mucosa (37.3%). Due to the absence of a pathological picture in the structure of the duodenum, as well as the important role of mucus in the formation of chyme and digestion, we consider the increase in the density of goblet cells and the effect of feed additives as a positive reaction. The study of histological parameters to assess the effect of various feed additives on the body parameters of birds is important, since it shows the functional state of the organs.